微生物轉(zhuǎn)化法合成天麻素等天然活性成分的研究.pdf

1、分類號——密級單位代碼!魚!!!學(xué)號20031104西北大學(xué)博士學(xué)位論文題目絲生塹鹽垡溘金盛苤座盍釜墨叢鯊：建成分的研究作者盎塞煎指導(dǎo)教師一塞絲鎣專業(yè)技術(shù)職稱教授學(xué)科(專業(yè))垡堂墨堡答辯日期20076學(xué)位授予日期二00七年五月StudiesonBiosynthesisofGastrodinandOtherBioactiveNaturalProductsbymicrobialtransformationAbstractGastrodina

2、ndsalidroside，themajoringredientofthewellknownChinesemedicinewerebiosynthesizedthroughmicrobialtransformationwhichisanewmethoddifferentfromchemicalsynthesisandtraditionalplantextractionInthisexperimentmutagcuesisofthestr

3、ain’optimazitionoftransformingcondition，extractionandpurificationofthetHlzymcwerediscussedinordertofindthemoreefficientandeconomicalmethodforgastrodinandsalidrosidesynthesisandofferanewchannelformicrobialtransformationof

4、otherbioactivenaturalproductsUsingthemethodofbatchcultureandrestingcultureRh／zop淞LN1andpseudomonasZ1，whichhavetheabilitytobiosynthesizegastrodinandsalidrosideweresreenedsequentlyfrommanyfungi，yeastandbacteriaaccordingtot

5、heirTLCandHPLCresultsTheoptimaltransformingconditionwasinvestigated：129restingcell(wetweigh0incubatedat28“Cfor24hoursina500raLflaskcontainin9120mLofmediumproducedagoodyieldofgastredin(16％)byadding23mg／mLsubstrate(finalco

6、ncentration)Theoptimalinitialpitofthemediumwas55Themaximumyieldofsalidroside(10％)wasacquiredunderthecondition：substrateconcentration15m#mLinitialpH7oincubatedat33“Cfor20hoursonarotalyshakerusingglucoseasairbOllsourceandy

7、eastextractorO眥hs04弱ni仃ogcnsourceThepreparativescaleexperimentwascarriedoutundertheidealtransformingconditiontheproductswereobtainedbyextraction，chromatographyandidentifiedonthebasisofits1H，13CNMRandElMSspectraldata0皿thc