土壤放線菌的篩選、鑒定及其發(fā)酵產(chǎn)物的活性研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文土壤放線菌的篩選、鑒定及其發(fā)酵產(chǎn)物的活性研究姓名：夏廣欣申請學(xué)位級別：碩士專業(yè)：生物學(xué)；生物化學(xué)與分子生物學(xué)指導(dǎo)教師：王相晶20120603Abstract詈鼉!!!曼詈鼉!詈!皇詈烹=!!曼苧!暑詈!!皇苧!詈詈皇皇詈皇暑墨!!!!苧!!!!暑!!曼!皇曼!曼曼!!!曼皇詈詈!皇皇!詈!曼皇!詈!!曼皇鼉!!ScreeningandIdentificationofActinomycetesfromSoiland

2、AnalysisonFermentationProductsAbstractActinomyceteshaveprovidedmanyimportantbioactivecompoundsofhighcommercialvalueThesesearcheshavebeenremarkablysuccessfulandapproximatelytwothirdofnaturallyoccurringantibioticshavebeeni

3、solatedfromactinomycetesThemajorityoftheactinomycetesarewidelydistributedinthesoil，SOthedevelopmentofactinomycetesinsoilisofgreatimportanceScreeningandidentificatingtheactinomycetesinthesoilbyseveraltreatmentanddetermina

4、tingtheantibacterialacticityoftheisolatedstrains，moreoverstudyingthefermentationconditionandstabilityofthefermentationproductsThedetailedstudiesasff)llows：Naturedryingandusingthreemethodssuchasmicrowawjirradiation，ultras

5、onictreatmentandenrichmenttopretreattheninesoilsamples，whichcameftomGuangdongProvince，LiaoningProvince，，ichuanProvince，ZhejiangProvince，HeilongfiangProvinceandShandongProvinceUsingselectiveisolationmediumstarchcaseinmedi

6、umandhumicacidmediumwih50Itg／mLpotassiumdichromatetoisolationandscreeningofactinomycetesAtotalof89strainswereisolatedfromsoilsDeterminingtheantibacterialactivityof89strainsbyplatemethodwiththetestedstrainsofGram—positive

7、bacteria25strainsshowedgreatantibacterialactivityagainstBacillussubtilisandMicrococcusluteus，andtheinhibitionzonediameterwereabove15mmStrainC1Wasshowedstrongactivityforbothofthesepathogensofantibacterialdiameterabove25mm

8、Determiningtheantibacterialactivityof89strainsagainstsevenpathogenicfungibyfilterpapermethod，andusingthediskdiffusionmethodtore—screenthe17strainswhichshowedbetteractivityinvitroTheresultsshowedthatthe17actinomycetesshow

9、edhighactivityagainstatleastfourofthesevenpathogenicfungi，including14strainswhichshowedgreatactibacterialactivitySevenstrainsshowedactivityagainstfivefungis，ThestrainC1showedactivityagainstsevenphytopathogenicfungis，andt

10、heinhibitoryactivityagainstColletou’ichumorbiculareandFusariumoxysporumreached8971％and7463％respectivelyOptimizingthefermentationmediumandconditionsofstrainC1TheoptimumfermentationconditionofstrainC1Wasasfollows：soybeanme