抗除草劑bar基因工程菌構建與草莓葉片再生體系的研究.pdf

1、安徽農業(yè)大學碩士學位論文抗除草劑bar基因工程菌構建與草莓葉片再生體系的研究姓名：朱秀蕾申請學位級別：碩士專業(yè)：果樹學指導教師：朱立武20080525AbstractForexploringefficientmethodfors竹awberry(FragaHaananassaDueh)anti—herbicidalbarGenetransformationbyAgrobacteriumtumefaciensthetriparentalm

2、atinghybridizationWaSdonetotransformeukaryoticexpressionvectorintoAgrobacteriumtumefaciensLBA4404TheproductsofmyceliumPCR，plasmidPCRandplasmiddigestedbyXbaIandBamHIenzymeweretestedbydectrophoresisTheconstructedprojectAgr

3、obacteriumwasculturedwithplanfletofstrawberryThestrawberrycultivarsToyonokasuitablycultivatedinAnhuiProvinceweretakenasmaterialtoestablelishasimpleandhigh—efficientleafregenerationsystemCallusandadventitiousbudinductionf

4、romleafandrootingof／nv／troshoothadbeenstudiedtoprovideafundamentforstrawberrytransgenicresearchThemainresultsgainedwereasfollows1、UtilizingtriparentalmatinghybridizationofLBA4404HB101andDH5aWasprovedthattheprojectagrobac

5、teriumwimeukaryoticexpressionvectorpBll21一barhadbeenconstructedsuccessfullybyelectrophoresisofproductsinPCRwithbacteriumcolonyandplasmidandinplasmiddigestedwithXbaIandBamHIenzyme2、Thecriticalconcen仃afionofkanamycinformor

6、talityofuntransgenics竹awberryplantletandcallusfromleafwas25mg／Land20mg／Lrespectively3、TheerificflconcentrationCefandCarbefficientforcontrolofprojectagrobacteriumgrowthwerebothat200mg／L4、Thecombinationof6BAandNAAconcentra

7、tioncann’tinduceadventitiousbuds，theyCanonlyinducecallusofstrawberryleaf5、ThebestculturemediumfortheadventitiousbudinducedfromleafWasMS6BA30m∥L2，4D02m∥LIBA03mg／LbyanalysisofSPSSsoftware6、Theoptimalrootingculturemediumsfo

8、rToyonokaWerl己1／2MSIBA01mg／L7、necriticalconcentrationCarbefficientforcontrolofAgrobacteriumrhizogenesR1601growthWasat500mg／L8、WhentheOD600ofAgrobacteriumrhizogenesR1601is06，itisthemosteffect011frequencyoftransformationKe