結直腸癌RHOA、RHOC基因表達及意義.pdf

1、青島大學碩士學位論文結直腸癌RHOA、RHOC基因表達及意義姓名：劉延軍申請學位級別：碩士專業(yè)：普外科指導教師：王海波20080424ExpressionandclinicalsignificanceofRhoAandRhoCincolorectalcarcinomaABSTRACTobjective：UsingrealtimefluorescentquantitiveRTPCRtofindthecorrelationsoftheexp

2、ressionofRhoAandRhoCwithclinicalpathologyofcolorectalcarcinomaToexplorethemolecularbiologicmechanismoftheoccurrencedevelopmentandmetastasisofcolorectalcarcinomaNewpathwayforearlierdetection，diagnosisandtreatmentofcolorec

3、talcarcinomacarlbeprovidedMethods：SampleswhichwereobtainedfrompatientsinGeneralSurgeryDepartmentofAffiliatedHospitalofMedicalCollegeQingdaoUniversityweredividedintofivegroupswhichincludethegroupofcoloreetalcarcinomawithh

4、epaticmetastasis、thegroupofcolorectalcarcinomawithmetastasisoflymphnode、thegroupofcolorectalcarcinomawithoutmetastasis、thegroupoftissuesidecarcinoma、thegroupofnormalcolorectalmucosa；whilethesamplesofmalignanttumorwereals

5、odividedintoseveralgroupsthroughhistolo百CclassesandclinicalpathologicstagesTodetecttheexpressionofmRNAofRhoA、RhoCwithrealtimefluorescentquantitiveRTPCRTheexperimentaldataWasanalyzedwithttestandvarianceanalysisResults：The

6、rearesignificantdeviationoftheexpressedquantityofmRNAofRhoA、RhoCindifferentclinicalstages(PO05)，differenthistologicclasses(P005)aswellaswithandwithoutmetastasisoflymphnode(P005)Therearenodifferenceinage、genderandthesiteo

7、fcarcinomaThelateroftheclinicalstages，themoreexpressionofmRNAofRhoA、RhoC，anditisthesamewithdifferentiationAlso，theexpressiongotupwithmetastasisofliverorlymphnodeObviouslytheexpressiongotdownwiththeadverseconditionsConclu

8、sions：ThereareintimatecorrelationsbetweenthequantitiveexpressionofmRNAofRhoA、RhoCandclinicalstages、histologicclassesandmetastasisofcolorectalcarcinomaThequantitiveexpressionofmRNAofRhoA、RhoCCanbeanindextojudgethemalignan