RNA干擾抑制Survivin基因?qū)θ烁伟〩epG2細胞增殖的影響.pdf

1、青島大學(xué)碩士學(xué)位論文RNA干擾抑制Survivin基因?qū)θ烁伟〩epG2細胞增殖的影響姓名：楊敬敬申請學(xué)位級別：碩士專業(yè)：腫瘤學(xué)指導(dǎo)教師：于壯20090524EffectofRNAInterferenceTargetingSurvivinGeneontheproliferationofHepatocellularCarcinomaCellLineHepG2AbstractObjective：Toobservetheeffectofthe

2、RNAinterferencetargetingsurvivingeneexpressiononproliferationinhepatocellularcarcinomacellHepG2andtoinvestigatethefeasibilityoftreatmentonhepatomawithRNAinterferencetargetingsurvivingeneMethods：Threedifferentsurvivingene

3、sequencespecificshRNAvectorspshRNAsurvivin一323／387／52wereconstructedTransfectionofshRNAvectorswasperformedusingtheLipofectmine2000liposomeItseffectsonthecellswereevaluatedbydetectingfollowingindexes：(1)Theexpressionofsur

4、vivinmRNAWasdetectedbyReversetranseriptionpolymerasechainreaction(RTPCR)ThespecificityofRNAinterferencewasanalyzed；(2)ThevectorwhichcouldsignificantlydownregulatetheexpressionofsurvivinwastransfectedintoHepG2cellsbyLipof

5、ectmine2000liposomeRTPCRwasemployedtodetecttheexpressionofsurvivingene24h，48hand72haftertransfectionThetime●’effectofRNAinterferenceWasanalyzed；(3)TheproliferationofHepG2ceilswasassessedbyMTTassayResults：(1)RTPCRshowedth

6、atthepshRNA—survivin一387vectortargetingsurvivingenecansignificantlydownregulatedtheexpressionofitsmRNAinHer’G2cellswhencomparingwiththatofthecontrols(P005)(2)RTPCRshowedthattheexpressionofsurvivinmRNAbegantodecrease24haf

7、tertransfection；andthemostapparentinterferingefficiencywasat48haRertransfection；at72haftertransfection，theexpressionrestored、(3)MTTassayshowedtheproliferationofHepG2cellsWasinhibitedConclusion：ThepshRNA—survivin387vector