四倍體葡萄誘導(dǎo)技術(shù)的研究.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文四倍體葡萄誘導(dǎo)技術(shù)的研究姓名：馬愛紅申請學(xué)位級別：碩士專業(yè)：果樹學(xué)指導(dǎo)教師：孫建設(shè)李紹華20050621StudyonInduclionofTetrapioidGrapesAuthor：MaAi—hungMajor：PomologyTuter：SunJiansheandLiShao—hua(DepartmentofHorticulture，AgriculturalUniversityofHebei，Banding

2、，China071001)AbstractlnductionsoftetraploidgrapeswereconductedwithcolchicinefrominvitroVitisviniferacv‘Jingxiu’‘RedGlobe’，‘Jingzaojing’，‘Rizamat’，‘ManicureFinger’，‘RedCocoon’，and‘YaIatiRosa’Ploidylevelsweredeterminedbyfl

3、owcytometryToexploretheeffectivemethodsandoptimaltreatmentsofinductiontetraploids，comparisonsofsoakingshootsegmentsincolchicinesolutionandaddingcolchinineintheculturemediumweredonewith‘Jingxiu’aloneTheeffectsofbothdurati

4、onofsoakingandconcentrationofcolchicineonefficiencyofinductionwerestudiedThelltetraploidgrapeswerefurtherinducedfrom‘3ingxiu’and‘RedGlobe’withanoptimaltreatmentFinally，fivecuhivars，including‘Jingzaojing’，‘Rizamat’，‘Manic

5、ureFinger’，‘RedCocoon’，and‘YatomiRosa’，wereinducedtetraploidsMainresultsareasfoIlows：1Thesoaked—treatmenthadincreasedefficiencyofinducingtetraploidsthanoneofaddinginmediumItwascharacterizedassimpleoperation，lesscolchicin

6、eusageandeffectiveinductionofbothpuretetraploidsandchimeradueiocellularinfiltrationeasilybysoakingOnthecontrarythetreatmentofaddiagcolchicinesintheculturemediumwithdifferentagesofinvitro‘Jingxiu’waspooreffectofinductiono

7、ftetraptoids，2TheeffectsofcolchicinenoinhibitingplantgrowthdifieredinconcentrationofcolchicineanddurationofsoakingstemfragmentsincolchicinesolutionTheinhibitionofplantletsgrowthwasenhancedwithsoakedtimeextensionandthecon

8、centrationincreaseDeathofplantletswasobservedwhentheconcentrationofcolchicineswashigherthan2000mgL‘orthedurationofsoakingwaslongerthan48hoursTileincidenceofdeathofplantletsmostlywashigherthan30％whentheconcentrationwashig

9、herthan40()0mgL1Thedeathrateof‘Jingxiu’and’RedGlobe’wasmorethan70％astreatedat4000mgL1for4d3Soakingshootsegmentswithcolchicineat2000mg‘L1for3to4dor3000mgL14000mg‘L～for2to4dinducedpuretetraploidsduringfirstregenerationAdes

10、iredrangeof167％to23。3％oftetraploidswasobtainedbysoakingatcolchicinesconcentrationof3000mgL～for3Io4daysHowevercnlchicineconcentrationsof2000mg‘L1orlowerandsoakingfor48horshorterinducedpuretetraploidsaftertwotofiveregenera

11、tionseventhoughtetraploidswerenotobservedduringfirstregeneration4Ploidyofplantletwasnotchangedthroughgrowing。butseparationofchimeraoccuffedduringregenerationSomemorphologicalfeaturesofleavesandrootswereobviouslychangedwh

12、ilechimerasbecameintopuretetraploidsThesechangeswerestronglyrelatedtoeithertheconcentrationofcolchicineordurationofsoakediothecolchicinesolution，511heefficiencyofinduetionvariedwithdifferenteultivatsTheoptimaltreatmentco