柔嫩艾美耳球蟲對馬杜霉素抗藥性的研究.pdf

1、分類號密級Y773859IjI蛾；蔓t夭等碩士學(xué)位論文單位代碼：10019學(xué)號：TS6074柔嫩艾美耳球蟲’對馬杜霉素抗藥性的研究Eimeriatenella：experimentalstudiesonthedevelopmentofresistancetomaduramycin研究指導(dǎo)教師：選建盛塾援合作指導(dǎo)教師：塞勵熬援申請學(xué)位類別：盔堂亟專業(yè)名稱：基趟盞醫(yī)堂研究方向：盞醫(yī)藥堡堂所在學(xué)院：動物醫(yī)堂瞳2004年10月Eimeriate

2、nella：experimentalstudiesonthedevelopmentofresistancet0maduramycinAbstractThedevelopmentofresistancebytheHoughtonstrainofEimeriateneltatomaduramycinwasstudied時seriallypassagingthestraininchickensmedicatedwithprogressivel

3、ygreaterconcenlrationsofdrugandtheSDS—PAGEtechniquewa3practicedtotestthehypothesisthatproteinvariationswereassociatedwithmaduramycinresistanceThemethodologyusedByWeppelmaneta1(1977)wasalsoappliedt0testWeppelmmaetalhypoth

4、esisthatresistanceresultfromselectionratherthanadaptationAfter33passageswith2mg／kgmaduramycinastheprimaryconcentration，alineofthestrainwhichbecameresistantto3mg／kgmaduramycinwasdevelopedSDSPAGErevealedthat：Milepeptidewit

5、hmolecularweightofapproximately2285kDawaspresentinthesporolatedoocystsoftheresistancelinebutundetectableinitsHoughtonparentsporulatedoocystsandapeptidewithmoleculm“weightofapproximately1866kDawashighlyexpressedintheresis

6、tancelinewhileanotherpeptidewithmolecularweightofapproximately93kDawaspresentinthesporulatedoocyslsoftheparemencystsbutundetectableinitsresistancelineThefailuretoselectamutatedlineagainstmaduramycinsuggestthatmuttplemula