黑龍江省南瓜病毒病毒原鑒定和品種資源抗性篩選的研究.pdf

1、東北農(nóng)業(yè)大學碩士學位論文黑龍江省南瓜病毒病毒原鑒定和品種資源抗性篩選的研究姓名：李鳳梅申請學位級別：碩士專業(yè)：蔬菜學指導教師：崔崇士2002.6.1型!!：堂垡堡莖塑些i!!!壅!生!：LStudyonValuationofPumpMnVirusinHeiLongjiangProvinceandSelectionofResistantPumpMnResourcesAbstractPumpkinvirusdiseaseispopulari

2、nlieiLongJiangprey／riceintheyearofhightemperatureanddrought，especiallyserjOUSinthewestareaThetypicalsymptomjsfoldandblistering，evendjstartjon，lopsidedi1】一]eaf，shortingplant：therearemotleywithlightgreenanddeepgreen，andsom

3、eofthemot]eyareyellowedPterid礎(chǔ)聊：eleavesovel刪type：theill—leafMsseriousmosaic，lops／ded，andthetissueoftheill—leafhasdeterioratedexceedinglywithslenderpteridophyteleaves，illplantisgreen—yellow，obviouslyshortenedinjoint，thewh

4、o】eplantseriouslyshortenedandgrowsthicklyInspectedandSurveyedinthemethodofserologic492％samplesareWMV一219896samplesareSqMV125％samplesareCgVin183SamplescolletedfromseveralareasofHLJpreyinee，andtheratioofthreevirusesisdiffe

5、rentindifferentarea，HarBinarea：533％samplesareWMv一2，204％samplesareSqMV167％samplesareoⅣ，OiQiHararea：478％samplesareW14V2，322％samplesareSqMV104％samplesareCMV，MuDanJiangarea：525％samplesareWMV2，133％samplesareSq^fv125％setup]esa

6、reDⅣ，JiaMuSiarea：432％samplesarem4、，一2，133％samp]esareSqMv，104％samplesare04VTheratioofWMV一2is4329卜_532％ineveryareaItiSobviousthatdifferentareashasthesamemainvirusresouree：WMV一2Thesampleoftypicalsymptomwhichwasseparatedinsi

7、nglespot，propagatingincucurbitapepo1hostjcanlnfect國cumissativuscitruilusvulgar_，sCucum／smeloWaxgourdcquinoaphaseoIuswlgans，cannotinfectPisumSatiFdmMgiutfBOSeLyeopersicumesculentumDutufastraeoniumNYoot／anotabacumTheJengho

8、fstringvirusparticleswhichwereobservedthroughelectronicmicroscopeis600—750nm塒衄plantleaves曲ich㈣e；㈣inedina$erslightsection，chloroplastmembranewascomplereandclear，bastcalparticalpiecesandbustealmaterJatpieceswereputinorderB

9、utpu叩kinleavesinfectedby怖tV2w婦chlotof)lastmⅢbranedissolvedbadicalpat／catandtRsica]materialpiecesweredisintegrate4basi∞]T]arLicleswereindistinetarldIlltindisorder：011蝴Iastswell磚andtherewereagrcatquantitydapasitmsswhichdes

10、troyedthechk口℃plastregulars七nocllge100seandTheresultofresis協(xié)ceofoutsideb。由is：6。℃ist聹出ad噸mI啪曲l聰thesurviwaltimeoutsidebo由is36days，thelimitationofdilutingis1礦TheresearchoftheselectionformⅣ一2expressedthatbeinginoculatedincot