乙酰膽堿酯酶與硫代黃素T體系檢測有機磷的研究.pdf

1、中南大學碩士學位論文乙酰膽堿酯酶與硫代黃素T體系檢測有機磷的研究姓名：劉偉申請學位級別：碩士專業(yè)：應用化學指導教師：黃可龍20070516中南大學碩士學位論文ABSl絮ACTABSTRACTOrganophosphatepesticidesthataremainlyusedinagricultureshowlowenvironmentalpersistencebutdisplayhighacutetoxicityTheirpresenc

2、einwaterandfoodposesapotentialhazardtohumanhealthIngeneralthesecompoundsinhibittheacetylcholinesterase(ACHE，EC：3117、participatinginnerveimpulsetransmissionTheinhibitionmechanism，beingveryspecific，hasledtothedevelopmentof

3、severalanalyticaltechniquesfortheidentificationandquantificationofsuchpesticidesonthebasisoftheinhibitionofacetylcholinesteraseInthispaper，basedonenzymeinhibition，anewsystemforfastreagentlessdetectionofpesticideshasbeend

4、eveloped，andAChE—ThioflavinTcomplexWasusedastheindicatortodetectthepesticidesInAChE—ThioflavinTcomplex，whenorganophosphatepesticideisadded，thepeak局‰decreasesharplyTheresultsshowthattheintensityoffluorescencequenchingat49

5、0nm0F4舳)islineartotheorganophosphatepesticideconcentrationCoPsThestudyofdetectingdemonstratethatstaticquenchingexistsintheAChEThioflavinTsupramolecularcomplexAdditionoforganophosphatepesticidetotheAChE—ThioflavinTcomplex

6、resultsinadecreaseinfluorescenceintensityat490nmduetodisplacementofthethioflavinTfromtheacetylcholinesteraseInthispaperthedetectionlimitsof787，and923刪forparaoxonandchlorpyrifos，wereachievedrespectively，whichprovedthatour

7、methodwasmoresensitivethanothersinChinaThedetectionsystemhasbeenappliedtothedeterminationofparaoxoninspikedvegetablejuices，andsatisfactoryrecoveryvalueswereachievedInthispaper，sol—gelbasedmembranewithAChEThioflavinTcompl

8、exasthebiorecognitionelementhasbeendevelopmentforthedeterminationofpesticidesTheanalyticalperformanceincludingreversibilityreproducibilitystabilityandselectivityhavebeenevaluatedandcanbeappliedforthedirectdeterminationin