外植體的母樹來源對水曲柳體細(xì)胞胚胎發(fā)生的影響.pdf

1、東北林業(yè)大學(xué)碩士學(xué)位論文外植體的母樹來源對水曲柳體細(xì)胞胚胎發(fā)生的影響姓名：馮丹丹申請學(xué)位級別：碩士專業(yè)：森林培育指導(dǎo)教師：沈海龍20060601東北林業(yè)人學(xué)碩：I：j學(xué)位論文AbstractSomaticembryogenesisisthebaseofgeneengineeringandselectionofmutation，andtheDreconditionofthatthesystemofchoicenesssenespropag

2、atlonlsapplledmtluenceot一●●●1’T，1rexplantsourcetreetosomaticembryogenesisinManchrianash(乃黜覷淞川口，z出^以廠fc以)，whichisthemethodofestimationtheapproachofpropagationManchrianashisoneoftheimportanttreespeciesinNonheastChinafortim

3、berproductionandlandscapeestablishmentThispaperisabOutsomaticembryogenesisandpropagationfromcotyledonsofimmatureandmaturezygoticembryosinManchurianashItwasobservedthatdifferentoptimalhomonecombinationswasnecessaryforindu

4、ctionofsOmaticembryogenesis|Dfvarioussourcetrees，alsO，thefrequencyOfsomaticembryogenesisaredifferent，suchassourcetree3，NAA2m∥LandBAO5m∥L，54％；sourcetree1，NAA15mg／LandBA05mg／L，18％；sourcetree4，NAA1m∥LandBAO5m∥L，16％；sobrcetr

5、ee2，NAA15m∥LandBAO5mg／L，12％CotyledonsofMaturezygoticembryosfrom37differentsourcetreeswereusedasexplants，thensomaticembryogenesisefficiency，MS1／2wasthebasemedium，combinnatedwithNAA5m∥L、BA2m∥L、caseinhydrolysate400m∥L、70∥Ls

6、ucrose、6∥Lagar、thepHwasamustedto58beforeputinautoclaveThereweredifferenceonsomaticembrVognesisfromcotyledonsofManchurianashindifferentsides；somaticembryognesisfromcotvledonsofdifferentManchurianashinonesidewasdifferent，t

7、hehighestfrequency(15％)andnoneofsomaticembryogenesisweretogetherGlobal、heartandtorpedosomaticembryoproliferatebestonMS1／2001m∥LNAA200m∥Lcaseinhydrolysate70∥Lsucrose、6∥Lagar、thePHwasadjustedto58beforeputinautoclave；cotyle

8、donarysomaticembryoscangerminateonthatmediumIncytologicalstudyofsomaticembryodeVelopment，bythewayofparaffinThecytolOgicalinvestigationOnsOmaticembryogenesisshowedthatthesomaticembryosfromz夕goticcotyledonsoriginateddirect

9、lyfromsingleepidermiscellEmbryogenicceUsweresmall，almostisodiametric，withdensecytoplast，largenucliandnucleolusNon’embryogenlccalluswerecomposedoflargercells，withirregularfigures，aHangedcompactlyorloosely，thincytoplast，li

10、ttlenucli10catedatonesideSomaticembryogensisunder、Ⅳentthesimilarcourse，passingthroughproembryo，heart，torpedoandcotyledonarystages。andSomatlcembrVoscangerminateintoseedlingsVascularbundledifferentiatedatheartembryostageth