30089.pha高產(chǎn)菌株的誘變選育及培養(yǎng)條件的研究

1、西北大學碩士學位論文PHA高產(chǎn)菌株的誘變選育及培養(yǎng)條件的研究姓名：何竹青申請學位級別：碩士專業(yè)：微生物學指導教師：黃建新20090611第一章前言StudyonMutationbreedingandOptimizeFermentationConditionsforPoly—hydroxybutyrateacid(PHA)OVerproductiVeStrainsAbstractPolyhydroxybutyrateacid(VHA)is

2、wellknowasakindofthermoplasticaliphaticpolysterthatissynthesizedandaccumulatedintracellularlyduringunbalancedgrowthbymicroorganismNowadays，PHAhaveattractedincreasinginterestasanalternativetopetroleum—derivedplasticsforit

3、ssimilarmechanicalpropertiestothoseofpolypropylene，aswellasitsadditionaladvantageofbeingcompletelybiodegradable，biocompatible，non—toxicityandetcAsanewkindofbiodegradableplastic，PHAhasbeenproducedbybiologicalfermentpresen

4、tlySofarmanyresearchfocusedonseparatingvaluablestrainswhichCanproducePHA，producingsrainsthroughcompoundmutation，andsudyingontheoptimizationalfermentationconditionsWeCompoundmutationtoproducing—srainsfromY4whichconservedb

5、yourlaboratorythenstudyontheoptimizationalfermentationconditionsofoverproductivestrainsjhordertoprovideanewoverproductivestrainforindustrializationFollowingresultshavebeenobtainedinthisstudyTheMutationstrainisattainedbyU

6、VandHeNelaserThedesiccatedcellmassfromBY120—30Was1329／Lenhancing435％：PHAyieldfromBY120—30Was70g／Lenhancing556％：PHAproductionratiofromBY120—30was530％enhancing84％Experimentizedtheshakeboricfermentationoptimizationandthebes

7、tsupplementedmediumfermentationconditionWasasfollow：25％canesugarO3％proteosepeptone，O3％yeastextract，O3％(NIh)2S04，O5％NaCl，initialpH=7，10％inoculationamount，liquidcapacity70mLin250mLflask，attemperatureof30“C，with220revolutio