大豆疫霉根腐病病原菌生物學(xué)特性及抗源篩選研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文大豆疫霉根腐病病原菌生物學(xué)特性及抗源篩選研究姓名：呂慧穎申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：作物遺傳育種指導(dǎo)教師：楊慶凱2000.5.1查苧奎些查蘭盔蘭筌曼堡塑塑墮曼里蘭塑蘭絲絲墨苧翌絲壟翌塞：竺!蘭：星StudyOilBiologicalCharacteristicsofPhytophthorasojaeandScreeningofGermplasmforResistancetothepathogenabstractPhy

2、tophthorarootrotofsoybeancausedbyPhytophthorasojaeisoneofthemostdangeroussoybeandiseaseThediseasewasfirstfoundinHeilon西iangprovinceofChinain199Iandthenithasbeella辯dousdiseasewhichhasseverelatentdamageofsoybeanproductioni

3、nHeilongjiangProvinceThisstudyservedasabasicresearchforresistancebreedingastudyoffactorswhichinfleuncedthegrowthofmyceliumofPhytophthorasojaeanditsz00sporesproducaonwascarriedoutbasedonR25ofPsojaeByusingR25andH4ofthepath

4、ogen，956varieties(orlines)fromHeilongiiang，JilinLiaoningProvincewereevaluatedforscreeningresistantgermplasm，whichcarlprovideresistantgermplasmandparentsfordisease—resistantbreedingprogrammeaswell∞providebasicmamfialsforb

5、asicresearchofresistantgeneticsresistantmachnismetcTheresultsofthisstudyshowedthatsuitabletemperatureformyceliumgrowthrangedfrom240Cto320Candtheoptimumtemperaturewere280cThepathogencouldgrowinmediumwithpHvalmrangingfrom2

6、to9butacidconditionwasfavourabletothegrowthofpathogenwhichdevelopedmuchfastwhenpHvaluewasfrom4to5Themyceliumgrewfasterwhileitwasincubatedinthedark111epathogenwasnotparticularaboutcarbonsousesitcouldutilizebothmonosacchar

7、ideandpolysaccharideTheoptimumnitrogensourceswerebeeftypetoneandasparagine，itcouldntutilizeammoniumm訂ogenverywellUnder250Cthemyceliumof8dayswereincubatedinthedistilledwaterfor72hours，suspicionsolutionofzoosporewithhighco

8、ncentrationcouldbeproducedYoungmyceliumproducedmorezoosporesthantheold，andacidconditionwasfavourabletotheproducaonofzoosporeTempemturewasanimportantfactorwhichinfluencedtheproductionofzoospore，buttherewasnozoosporerrcedu

9、cedunder00C、350C日∞appropriatetemperaturefortheproductionofzoosporerangedfrom200Cto300Candtheoptimumtemperaturewas250CThesoybeangermplasmfromthethreeNortheastProvinceswereevaluatedforresistance幻Psojae23varieties(orlines)，