無膠篩分毛細管電泳相互作用分析及毛細管電泳手性拆分.pdf

1、重慶大學碩士學位論文無膠篩分毛細管電泳相互作用分析及毛細管電泳手性拆分姓名：黃芳申請學位級別：碩士專業(yè)：分析化學指導教師：夏之寧20040430重慶大學碩士學位論文英文摘要ABSTRACTThepaperismadeupoftwoparts，thefirstpartisnongelcapillaryelectrophoresisinteractionanalysisSincemigrationtimewindowistoosmall，c

2、apillaryzoneelectrophoresispeakshiftmodelCan’texactlymeasuretheinteractionbetweendrugandproteinwhichhavesimilarmobilitiesThemechanismofmolecularsievingofentangledpolymersolutioninnongelcapillaryelectrophoresismGCE)wascit

3、edinthepaperInordertoexpandmigrationtimewindowentangledpolymerwasusedtochangethemobilityofproteininthebuffersolutionThemodelofinteractionanalysisbetweenproteinandligandpossingsimilarmobilitieswasestablishedbyNGCEThismeth

4、odfllrtherdevelopedtheanalyticalmethodsofcapillaryelectrophoresisinteractionanalysis1SincemobilityratioisindependentofviscositiesitWasintroducedtothecalculationmodelofcapillaryelectrophoresisinteractionanalysis，inorderto

5、efficientlyeliminatetheeffectofviscositiesofentangledpolymersolution2Linerpolyacrylamidewasusedassievingpolymertodeterminethebindingconstantbetweenchlorpheniramineandbovineserurualbumin(BSA)IncontrastwithNGCE，capillaryzo

6、neelectro。phoresisWasalsousedtomeasurebindingconstantbetweencMorpheniramineandBSATheresultsoftwomethodswerealmostconsistent3DextranwasusedassievingpolymertodeterminethebindingconstantsbetweenIndomethaein，andBSA，Irbesarta

7、nandBSAseparatelywhichweresimilarwithmobilitiesThesecondpartisehiralseparationofcapillaryelectrophoresisTheenantiomericseparationsofchlorpheniramineandsibutraminebycapillaryelectrophoresisusingp—cyclodextrin(p—CD)astheeh

8、iralselectorwerestudied1Bindingconstantsbetweentheenanfiomersofchlorpheniramineandp—CDwerestudiedatdifferentpHTherelationshipofresolutionandbindingconstantsWasdiscussedSincetheconcentrationofBCDatwhichthediscriminationof

9、enantiomericmobilitiesWasmaximalwasinconsistentwiththeconcentrationof13CDatwhichtheresolutionoftwoenantiomersWasoptimal，thepaperdiscoveredtherelationshipofresolutionandthemobilityoftwoenantiomers2Theenantiomericseparatio