川芎嗪對(duì)糖尿病腎病大鼠腎組織TGF-β1和CTGF表達(dá)干預(yù)的實(shí)驗(yàn)研究.pdf

1、成都中醫(yī)藥大學(xué)碩士學(xué)位論文川芎嗪對(duì)糖尿病腎病大鼠腎組織TGFβ1和CTGF表達(dá)干預(yù)的實(shí)驗(yàn)研究姓名：韓斌申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中醫(yī)內(nèi)科學(xué)指導(dǎo)教師：謝春光200705成都中醫(yī)藥大學(xué)碩士學(xué)位論文2ABSTRACTObjective：ByobservingtheeffectofLigustrazineonthepathologicalchangesinkidneyorganizationofDiabeticNephropathyratsand

2、theexpressionofTGFβ1、CTGFinvestigatethemechanismofLigustrazineinpreventingdiabeticglomerularsclerosisandinterstitialfibrosis.Method:AftercreatingtheDNmodelsofSDratsdividedtheanimalsintofourgroupsrandomly:includingmodelgr

3、oup，westmedicinegroup，Ligustrazine(highandlowdosage)groups.TakeanotherSDratscutrightkidneyasnormalgrouptotalfivegroups.Theratsoftreatinggroupsareaffusedinthecorrespondingmedicinetheotherareaffusedinthesamevolumewaterduri

4、ng12weeks.TestUAERinthe4th8th12thweekrespectivelyandmeasurthelevelofBUN、Scr、TC、TGinserumofeverygroup12weekslaterthenkillthemcuttheleftkidneysfixobservethepathologicaltransformationofrenalglomerulus.TheCTGFandTGFβ1aredete

5、ctedbyimmunohistochemistryandstatisticalanalysis.Result:Comparingwiththenormalgroup，thelevelofTG、TCandUAER、BUN、Scrofthemodelgrouprosecontinuously(P＜0.01；P＜0.05).TheexpressionofCTGFandTGFβ1inrenalorganizationraisedsignifi

6、cantly(P＜0.01).LigustrazinehighdosagehadtheroleofgettingTG、TCandUAER、BUN、ScrdecreasedandstepdowntheexpressionofCTGFandTGFβ1inrenalinterstitial(P＜0.05).Conclusion:Ligustrazinecoulddecreasethedrainageofuricproteinpreventth

7、erenalinterstitialfibrosispostponetheprocessofDN.TurningdowntheTGandregulatingtheexpressionofTGFβ1、CTGFinkidneymaypossiblybeoneofitsfunctionalmechanisms.Keyword:LigustrazineDiabetesNephropathyThekidneyOrganizationTGFβ1CT