青霉TS67菌株發(fā)酵條件優(yōu)化及抗菌物質(zhì)的分離純化.pdf

1、Y“z9805、、、H婁號天津商業(yè)大學碩士研究生畢業(yè)(學位)論文姓名：李海峰年級：2004級專業(yè)：發(fā)酵工程研究方向：微生物資源開發(fā)與利用論文題目：青霉TS67菌株發(fā)酵條件優(yōu)化及抗菌物質(zhì)的分離純化導師：王索英教授完成日期：2007年3月天津商業(yè)大學ABSTRACTThemalinefungiTS67whichhasbroadspectrumresistancetopathogenicfungiwasscreenedInthispaperc

2、ulturemediumandculturalconditionsandthepurificationoftheactivesubstancewasresearched，andthemethodsofpuriflcationbyadsorbingofmacroporousadsorbentresinwereestablishedTheresearchofculturemediumandculturalconditionsformarin

3、efungiTS67activesubstanceshowedthatnitrogenSOurCepresentedthemostsignificanteffectOntheproductionandthatglyceroladditionwasbeneficialItwasconcludedthatthefermentationactivitywasincreasedby31timesto1230u／mlundertheoptimal

4、conditionsTheoptimizedmediumWascomposedofglucose59／L，glycerol59／L，peptone39／L，yeastextract10g幾，andtheoptimizedcukuralconditionswasthattheinitialpHvalueis80。inoculationquantityis8％，culturaltemperatureis28℃，andfermentation

5、periodis96hoursTheresearchofpurificationofthemarinefungiTS67activesubstanceappearedthatresinX5isthebestadsorbent；thebestpHvalueis5：theeluantis50％ethan0150％methanolissuitableforelutingthepigmentandimpurityabsorbedbytheres

6、inadsorptionsaturationresinWasflushedbydistilledwaterthenwaselutedby50％methanolforremovingthemostofthepigmentandimpurityLateradsorptionsaturationresinWaSelutedby50％ethanolattherateof4mL／min，thenthehigberactivefermentatio

7、nWascombinedandconcentratedAtlast，concentratedfermentationwasstandingfor49handthegruffcrystalwasobtainedThethermalstabilityexperimentshowedthattheantibioticWasinstabilityunderthe50“CorhighenTheacidalkalistabilityshowedth